It automatically finds restriction sites, methylation sites (dam and dcm), ORFs, and potential primers. pDRAW32 is a basic, yet comprehensive, DNA analysis program that can be used for virtual cloning, annotations, and analysis. Recently updated, pDRAW32 is a free Windows program (but it may be run on a Mac with a porting software such as Wineskin). This includes two tried and true favorites, as well as some newer ones that may not have made it onto everyone’s radar yet. To help get you out of a molecular biology rut, I’m going to introduce you to a few DNA annotation/plasmid mapping programs. Sometimes though, we don’t want to spend the extra time learning a new program even though it might make our lives easier in the end. Don’t get me wrong-this is not necessarily a bad thing! Many times we don’t go looking for new software because the old stuff suits our needs. If you are like most molecular biologists, you probably use the same software your colleagues do-usually it is either the stuff that gets passed down from grad student to grad student or the one licensed software your lab pays for. A quick Google search brings up dozens of hits – but how do you know which one to use? Plasmid mapping and DNA annotation software is pretty abundant these days.
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